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CRIMIC CR71693

Gene Gem2

CG10419

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:19099123..19100363	75F7-75F7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACTGGCAACATGTATGAAACG	GATGAGCATCACAACAGCTGCGAT

Images

Construct Design Sequences

sgRNA sequence	TTCCAGGGGCACATTGCGTACGG
sgRNA2 sequence	TAAAAAGCCAAAAGCTACGCTGG

 

left_200bp	right_200bp
ATAAAACAAGCACTGAAAAAAAGGGGCAAAGCGATGATTTTGAAATATTTCTCACATATTCTAAACATAATTCACTTAAAAATAAATTAAAAATAGTTGTAAGTTTATTATGAACCTCATTCCAATTTGAAATTACCGTTATCCCACTTTTTGATGTTGGCAACCCTAGAACACTACTCGGTATTCCAGGGGCACATTGCGT	CGCTGGTAATTTTGTTTATATAATTACTCTACGTTTAGATACTATTTTTATAGTTTTCTGTGGCTGAATTATATTCTTTTGGTTAGAAAACAGATTTATTTATGCATATCAATCAAGAGACAACATATTGTTAATGAACCTAAAAATTAGATCGTAAAACATAAAAGTTTAAATATAAAAGTAATGATAATATCTTAATT

 

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