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CRIMIC CR71694

Gene Gem3

CG6539

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:10459081..10463101	67E3-67E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCAATTGGTTGGAGTGCTTGGT	GTTCAATTGGTTGGAGTGCTTGGT

Images

Construct Design Sequences

sgRNA sequence	AAAAAGTGTAAATTGTAACAAGG
sgRNA2 sequence	GAGACTGATACAGCCTCTCTTGG

 

left_200bp	right_200bp
AACAATTATACTTTATTTCTGTATGAAAATAGGAAAAATTTAAACATTTCTCTAAAAACAAATGATATTTTGTTACTGTAGTTTTGGTTCTGCGTAAATAGGTTTAATATTCACGAATAATTCGTTTGTAGTATATTTTAGTATATAATTATTCGCGTATGATGGATTTTTTGGCCGGCAATAAAAAAGTGTAAATTGTA	GAGGCTGTATCAGTCTCTTTCCTTACAATTGTATATCGTAGCCAGTTAAACCGTTTTCTTAATTAGAATACTCAATGTCGAAATAGTTGATAGCATGTTGGATACCATGAATGTTTTTTTATGCAAGCAATAAATTGCAGAATTTGTTACCAAAAAAATAAATGAATTAAAGCAGGAAACTAACTATTTTGAATTTATTA

 

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