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CRIMIC CR71697

Gene HBS1

CG1898

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:1862881..1865240	62B7-62B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACACATCATTGTTCACACAAGGC	GTCAGCCGCATATTCACATTTTCG

Images

Construct Design Sequences

sgRNA sequence	AATAAAGGTGCGTGCAGGTGTGG
sgRNA2 sequence	CGCGTGATGCTTCGCGTGGCGGG

 

left_200bp	right_200bp
CGACCTTATAACATATTTGAAAAATGTGCAGATTTTAAAAATAACAAATTATTAGATTCTTATGTCAATTGATTGATACATGAAGCAAATCAGAACAGTCGCCTCTCCGCTAGTGGCAATACCTTCTAACCGTACTATTGTACTAAAAACTCCTGGTCAAAGTGCCGCAAAATACCAAACCTCTGAAAAGGCAGCCACAC	GGCGGGAGTCACCATTGCCGCCGGAATGGTCACCAAGATCCGCTAGGTCGATGACCGCGCTGGATTTTGTACGAAATTTTAGTTAGATATTAACTTTTGTTGATTTCCTAAATATACATAGTTTATATGAATTGATTAAATCGAAAAGACGCTGAAGCAGTGGAAGAGATTTCGGTCTGACCATTTTAGCGGTATCTTAA

 

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