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CRIMIC CR71698

Gene chico

CG5686

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:10242512..10247078	31B1-31B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCATTGTCCTGATTTCAGGAGCTA	CGGACAAGAGAAGTACACTTGGA

Images

Construct Design Sequences

sgRNA sequence	TGTTATCGTTATCGCAGCATAGG
sgRNA2 sequence	TCCTCCGGGCATGCGGACACCGG

 

left_200bp	right_200bp
TTTAGTTACCGATAAAAACATTTCTAAAATACAAAAAATTTAAAATGATGATTAAATAAAAAACTGTTATACTAAATTTAAACGAAACAAACGGTCATTCGATAACTCAATTAGTATCGAATAAGCCGGCGTGTAAATCGGGCTGGCAACTCTCACCGGTGTAGAGATCGGGATGGCAACTTCGTACTGTTATTCCTATG	TCCGCATGCCCGGAGGATGGGAATACCTATGCGAAAATCGACTTTGACCAATCCGACTCCTCTTCCTCCTCATCGAACATATTTAATACGTAAAGTTTTGAAATTTATGACCCTATCCTATATATATGATTTGTTTAATATTGTACATTTATTGTAAATATTCTGACAAGCAAAGCTTACAATTTTGGATGCTAATAAAT

 

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