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CRIMIC CR71700

Gene CG5776

CG5776

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:12700949..12704451	33F3-33F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTACCAACACTGTCAGTGATGTC	CTGTCTAAACACCAAAGTCGACCC

Images

Construct Design Sequences

sgRNA sequence	ATGAAAACAAAACGTGCAACCGG
sgRNA2 sequence	AAGTAGATTAAACAACTCAGGGG

 

left_200bp	right_200bp
AATTTTAATTATTTATAACTATCGAATATTTAAAATATTACGCAATGAAATTAATATTAACAAGCAATCCGTACGCCATACGATTATTATCTGTTTAGCTATACCAAGATCTAGCTATTTTCGATCAAGATCTGCCAGAGCTTGCGCTCAATTTGCCATTCACAACGAACGTTTCCACCCACAATGAAAACAAAACGTGC	CAGGGGAACCTTGACTTTAGAACTATTTTAAGATTAATTGTGAAGAATCCGCAAAACAGTGACGATAAGATTACCGAAAGCAAATCTGCTTAATTTGTTTAAACAAATTACCATGTTATTAGCTCTCCAAAATCTGTTCACTCGATCAGCCTTCAAATCGGAGTTATGTATGTAGTTATGTAAGTTATGTAAAGTAGAAC

 

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