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CRIMIC CR71701

Gene Gle1

CG14749

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_KG4	II:8607900..8630186	44D5-44D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCATTGAATCTCTTGCTTGCATTG	TGGACGATGAGGACGATGACTAGT

Images

Construct Design Sequences

sgRNA sequence	TAGTAATCGTATTAAGACACTGG
sgRNA2 sequence	TGGCCAAGTTCCTGCGTGAGCGG

 

left_200bp	right_200bp
cTTTAcAAATTCGAGTTCGGAATCGCTGGACCAAAGCATTTCAATTCGTAAGTAAGACCGTATCCCCTTAACAATAACGGAATCCTGATTGCCGCCCAATGCGAGTAATATTAAATTGAGTTGGCAGTCTTATGCTATATTTTTGTTGATTAAAAAGTGTAAAAACATATTCGAAATTAAAAGTAGTAATCGTATTAAGA	GAGCGGCAGATTGCACAGGCTGTCGGAGTTTTGCCTCCAGGATTTTGGTAGGCATGCGATGATTTAGTTAGTTTTGTTAAGTGTGTGTATTTTTATAAACAAAAGTGATTATTGTAGTAATGGGACGTGTATTCATTCAGTAAATAGAGAAAACTTGCTTGTAAACGCATACGTTTCATTTCCAGAAATGCAACAATTAA

 

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