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CRIMIC CR71711

Gene CoRest

CG42687

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_pM37_p1	:..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
		CCACTTATCTGTACCGTCTCCCTT	AAGCTCTTCCCGTGGAACTGGAA

Images

Construct Design Sequences

sgRNA sequence	TGTCTTAGTATTCTATTCGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'	Amplicon 3'

 

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