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CRIMIC CR71712

Gene CG31875

CG31875

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:9995561..10002736	30F5-30F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCTTAGGTAAACCCAGGAATGGG	TTTTGGTTGGCATCAACAGTTG

Images

Construct Design Sequences

sgRNA sequence	TCTACATGAAGATAGCACCGTGG
sgRNA2 sequence	TTGGAAGATACAGTCCATGTGGG

 

left_200bp	right_200bp
GCCGATAGGTTTTGAGATAAATTAAAATTCATACAACAAAATATTATGTTTATTTTTTGAATGAGTTTATAATTTTAGAATTTAGACAGTGACCCGATCCATTTATTATATTTCCTATATTCTATGGGATATTTTCTTCGTAAGAGTAGTCAACACTGAAACTAGACGTCAAAACACAACACTGGATTTTTTGCCCACGG	TGGACTGTATCTTCCAAGCGCATGGTTATTTAGATTATGATAGTATTCAAACTATTCATTGACCAACGATTGATATTAATCACTATCTATTCAACGTAAAATGCTTTAAGTGTTATTTGTATTTTTCTAAGAATTCACGTGTTCAAAAAAACCACACAAAATAATAGGTATTTAAATGTATTCAAGGTCATATTGAAATA

 

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