CR71717

CRIMIC CR71717

Gene Set2

CG1716

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:13413242..13421024	11F4-11F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTGTGACCATGCCAAGCAT	CAGCTCTCAGCTACTGTACGCTTT

Images

Construct Design Sequences

sgRNA sequence	GGACTGCGCGATTTAGCATCCGG
sgRNA2 sequence	GTTAAAGTTCTAGTTCCGTGTGG

left_200bp	right_200bp
AACAAGAAAGAAGAAGACCGCCGACGGAGAGAAAAATCGAAGTTGCATTGGTATTGGTGTCTCGCCGTGTGCATGTGACAGCAAGTGCAGTTAGTTTCTAACTAATCGTTTCAGGGATTTGGGATGGCACGCAAAGAGTAGATATCACTGCAAATCGGACACAAATCTGCATCCCCCGTCGTTGGACTGCGCGATTTAGC	GGAACTAGAACTTTAACTTTAGATACGAATTTAGTTAAGCACATTCGACCCCTAGGATGCGATCTACCCCTCGTAGTGGGTAGCGTTTTAAGTGGGGGCAGCCAAATTGTACAGTTAATGAAATGGCGTAAGATGTAAGTGGAAAAATTCACAATACTTTTTGTTTCTTAAAATTGTCTAAAATTTCTAATAAATTCGCA