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CRIMIC CR71718

Gene CG4565

CG4565

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:10852724..10853644	86C7-86C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTCACCATGCAGGGGAAATCTGA	CCGAGCTGCGAATGTATCAC

Images

Construct Design Sequences

sgRNA sequence	ATGTAAACAAACTTTAAAAATGG
sgRNA2 sequence	GCAAGTACTTACATATACTTAGG

 

left_200bp	right_200bp
GCAAAACGGTCGAACTGGAACTGCAAGGATATACAAATGTGTTGCATCATAAAAAATAAACAATTTTCTTCCGAATAAGGGAACATTTTTAAATTCAGAGTGTGACCGAGGCAATAAAGTGTAATATTCTTCAGAAAGCGCAACATCCATTAAATGGGGAATCAATTCTCGTACTAGAATGTAATGTAAACAAACTTTAA	TATATGTAAGTACTTGCCACTCAGAGTTTGTTCTGTACTTGGTTTTCCAGCGGAGGTAGTTTTTCATAAATCCATTGCTGCAGATCCGTGATGGAGGCAGTTTTCAGTTCGTGCAGTGTGTTCCTCAATGGATGAAATGTGCCCTTGACACCCAGTTTCGTTAGGTTCTCGAAGGTTTCCAGACCCCAGTCTTTAGGTAC

 

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