CR71721

CRIMIC CR71721

Gene CG5828

CG5828

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:5093976..5095910	25C6-25C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTATCGATAGTTGCAATAAGACTGTTC	CGTAATCCACACCATTCGGTGCTT

Images

Construct Design Sequences

sgRNA sequence	TTATTATGGCAGGTGTGACCAGG
sgRNA2 sequence	CGTCGTCTGCAAGTTCGAACCGG

left_200bp	right_200bp
TTTATTTTGATgTATATTCTATATATACATAAAATTTTGGGATTGGTACAAAGTAGTATTTTCGAACTGCCCGCCCAaTTATCAGaTTTGTGAAATTAACGGTATtATTAAGCACGATAGTCGGCGAGTAATCGGCTTGCACAAAACGGAATACATTTAGTTATCGATTGCATATCAACTTCGCGTGCAATGCCCCTGGT	AACCGGCTGCGCCCAGCTAGTCCAGGAGCCACTACGTTCATTGTTATCCCTTGTTGACAAACAAACGATATCCCCGGTCCTTGTTCAGTTTGAAATCATTGTTACCGCCACCCAAAACCCACAACCCCCTAAACAATCCCATCCCTCAATCTACCGATGAGATTCATATGTGTATAAATGTAATCTTAATGCAAGTGCTT