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CRIMIC CR71724

Gene Beta-Spec

CG5870

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:17657372..17669371	16B10-16B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTTTGTCTGCCGTTTCGATAG	ATGCATACACGAGTTGGTTTCCTCAA

Images

Construct Design Sequences

sgRNA sequence	TGAAGGCAACACATTCGGTTTGG
sgRNA2 sequence	TCTTGCTTATGGTTGCGTTACGG

 

left_200bp	right_200bp
TCGCTTTAAGTCATAGTGACAATTACGATCGGACATTCTAGTGGTGACCCCGGCGACCCGCGTGACGCCATCCCATGCCATGGCAGCCCAAACCCAAACCCAAAACTGACATAAATGAATCACCAGCCGCTGCTGACAATTATCTAACCGATTTTCCCACCGCCTCTTCCAGTGATTCTCTTCTGAAGGCAACACATTCG	CGCAACCATAAGCAAGAGCAGCTAATTAAAACAAATAACAAATAACATGAAATGATATAATACAAATGCAAAACGACAACAAATTATACAGCAAAAATTAAGAAGAAGCAAATATATATATTTTTTTTTCATATATTTAAAACTTATTTCGTAATGAGAAAAAAACAAAAACCGACAAAAACTTGCAAGAGAAGAACGAG

 

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