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CRIMIC CR71727

Gene Argk2

CG5144

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:9047437..9048724	66F3-66F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACAATGGTTTGGAGCCGTGC	TTGTAGCTATGGTGTGGAGCTGCT

Images

Construct Design Sequences

sgRNA sequence	AATTTGGAATGACACCGGCCAGG
sgRNA2 sequence	TATTTATTTAGAGTTCATCACGG

 

left_200bp	right_200bp
ATATCAAAATTTACTTTATACTTTATTTATTTCAAATTTACTTGTACATAGATTGAGTCTTTGCTaAGCAATCTaCTTTCCGATATCTATTAAAGTGGAGTTTctAACCGTCTGTcTTTTGTTCGTACTAAATGGCGTTTTACTAGATTTATTCTAGCCATTTGGTTTAATGTCACTTTCTAACTTAGGGTCAGCCTGGC	TGAACTCTAAATAAATAATAAACTAAATATTTGTATACCCTATATGTCCTCTATATCCATTGAATACAAAACATTCATTGAGTTTTAAGTTTTTTTTTTATTTTTTTTTTAATAATTTTAATTCTTTTATAAACTTAAGACTTAAATAAAAGTATTTAAAGTTCCTTTTTTTGTTTTTGCTTGACACAGATGTCAAAATG

 

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