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CRIMIC CR71731

Gene CG7755

CG7755

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:16281630..16282946	53A5-53A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGCCTTGGTCACACCAATCTG	GGGCACCACGTATTTCTGATGCA

Images

Construct Design Sequences

sgRNA sequence	TACTTAGCAGATACATTCCTAGG
sgRNA2 sequence	TGCCATAAAATATATGATATTGG

 

left_200bp	right_200bp
GCTTCATTTAAAAACAAGAGAGAATTCTATAGTTAAGTGCATCGACTATCAGATACCCGTTACTCAGCTGAAAAATAGCAATAAAAATTTCATTCTTTAACTTTAATTTAGGAACTCGGACTTAGGAATAGGATCTCAAAATATTTGTGTATACAGTTGTGGGGGCAATTTGTGTCACTTGCATACTTAGCAGATACATT	TATTGGGAAAAAAAAACTCTCATTAAATTGTCTGTGACCATTATTTTTCCAAAATAAAAACCAACTATTTTTAGTTTTTCTCGAAATATTTTTTTATCAACACATAACAATTTATTAGCTTACAACGAACAATAATTTTAAGGAAAGTTGTCAGTAGTTGCGATATTTTTGGGAGCTCTTCAGCTAAAGTTTAAGATTAA

 

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