CR71734

CRIMIC CR71734

Gene Ipk2

CG13688

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:573033..574264	21E2-21E2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATCGGAGCCGATAAGCTCAAAG	AGACGAATCATGCCAATGCGCATC

Images

Construct Design Sequences

sgRNA sequence	CACTCTTGGCCATGACGGCGCGG
sgRNA2 sequence	ACTTCTACTACATCTTTGAGTGG

left_200bp	right_200bp
TGTCGCCTTGAAATGATTGCATCTTGCTAACATGTTCCCGCCAAAAAGGCCAGACCCTGGCGGGTAGCCAGCCGGTTAGCCATCACTGGTTCAGAGCGGTAAGTGCGCGCTACGTTTTCTGTGCGACATTTGCAAAGGCATTCCGATCGCCGCCTTCAGAGGTGCTGCTCCTGGACCAGACTACTGGACTACTACCGCGC	AAAGATGTAGTAGAAGTTCCAATCTCATTGCAATGAGACACTTTTGTATTATCGTCTAAGCCAAATGATTAGTCCACAGTAGATACCCATCCGTTTAAGGAACACGGGCGGAGCTACTCTAAACGTAGAATAAAACCAGATTTTTTAGAAATTGTGTATGTACTTCAAATCGAATTTGTGAATGGGAATGAGAGGTGCGA