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CRIMIC CR71754

Gene Wwox

CG7221

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:7999670..8004282	28D3-28D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTTTAGCACCTAAGTTCGTTGAC	GTACCAGGAGAAGGAACCACTCAA

Images

Construct Design Sequences

sgRNA sequence	TTGAAGATATCGATAACATGCGG
sgRNA2 sequence	TTTATTTGCATTGCACTTATTGG

 

left_200bp	right_200bp
ACAGCAGGCAGATTAAACAATGCCCACAAATGTTCAAGGCGCTAAGTTTCTACAAATCATTTAAATTTAAAAAACAACCGCCAATCAACCGGTTGCGGTTAAAAGCTCTGTTAAACGGATATGCTGATTATTAGCGGGTAACATGTTTCGTTTAACTTAACAGCTGTTCATTTAGTCGGCAGTTATCGCTTAAGCCGCAT	AGTGCAATGCAAATAAACTTTTGTACGTTAAGAATATCATGTATTTTCTTTTTACACTAAACAAGTATACATTTTGATTTGTTTTTAAGTCTTAATGGCTAGCATTTAAATAACAGTCTAAGTACTAATTAAGGAACTCTTCTTTTGGGCACTAGAAATCGGAGACGCGTCCCTTGCGCTCCCAGTCGCCGTAGCGTGTG

 

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