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CRIMIC CR71756

Gene chb

CG32435

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:21172853..21183278	78C1-78C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGTCATTGCTGTGCAGTTCCTTG	AAGTGAGGATTGCGGAGTTGGCTA

Images

Construct Design Sequences

sgRNA sequence	TCTATTCGTCCTTCGTTCTGCGG
sgRNA2 sequence	CGGCATCGTCGTCATGATTGCGG

 

left_200bp	right_200bp
AAAACATATAACATAATATACGCCGCTCCGAAATTTGGTTAGTTGCAGCCATCAATTTCACATTTAATGGCCCTTCAGTTTTACGGTGCCTGCATCCTTGCGCCAAAATTTGACCCTCACTCATACAACTTTAATTTAATTTGGCAAAGATTGTACCTGCATACACTAACACCAATCCTGTTTTATTACAGTTGCCGCAG	TTGCGGGAGCCCTTAATAGGATTCCTGCATCAGCACCACCACAAACAAGACACAGACGCGGTTGCTTCCCTCGGCCTGAGAAGGAAGTGAGGAGCGGCGGACATTAAATAATATATTATTACATTAACTAATTATTTATAACTAATTATTACCGATCATCCGTTACTTGTGTAACTGCTCGATGCATATATTCAGCAGAT

 

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