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CRIMIC CR71786

Gene Rrp1

CG3178

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:2969773..2972508	23C3-23C4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGCGTTTAATGGCGTCCTCATT	GCTGTTTACTTGGTGACTCCCCAT

Images

Construct Design Sequences

sgRNA sequence	TTTGCAGAGCCACTGTCGCTTGG
sgRNA2 sequence	AATAAAAACCATATATGGAGAGG

 

left_200bp	right_200bp
CCATTTTGTACTACTAATGACGTCaAACTATTTGTACTACTAGTGACGTCACAATGTGCAATATTTGCTCCACAGTtGTGTTTTTCCAAGGAAGGCACACAAATAGTGTTGTAAGCAGACCAAAGTCAAGCCGACCGGCTTGCAAAAATCGCGTTGCCCGCGCAGTTTTCTTTTTGTTGATATCGGGTGCTGAACCAAGC	CATATATGGTTTTTATTGTATTTGATTATCCATCGCTGTAGAAATTGTACGTCCCTATCTAATCATTTCAAAATGCCATTACTCTATTACATTCTGTCATAGATCCACCATCAAAATTCGTATTTTTTTAATAAAGTGAGCTACTGCCCCACGTTCGAACTcTGTGCTCAAATTATTGGCCAAAAACGGTTGGATTTAAA

 

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