( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 28
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1	0.0002	358192	{main}( )	...\info.php:0

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( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 33
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( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 34
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( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 39
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( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 41
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CR71790

CRIMIC CR71790

Gene BEAF-32

CG10159

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
		GCAGCACTACGATATTAACGAGGAG	CATCGTTCTGCTCGTACTCCT

Images

Construct Design Sequences

sgRNA sequence

CATAACCCAGAGATGGGCGCCGG

sgRNA2 sequence

( ! ) Deprecated: htmlspecialchars(): Passing null to parameter #1 ($string) of type string is deprecated in C:\wamp64\www\pscreen\crispr\phpMyEdit.class1.php on line 1168
Call Stack
#	Time	Memory	Function	Location
1	0.0002	374912	{main}( )	...\construct.php:0
2	0.0003	390224	phpMyEdit->__construct( $opts = ['hn' => 'localhost', 'un' => 'karen', 'pw' => 'flygirl', 'db' => 'crispr', 'tb' => 'construct', 'page_name' => 'construct.php', 'key' => 'ID', 'key_type' => 'int', 'sort_field' => [0 => 'gene'], 'inc' => 50, 'options' => 'ACPVF', 'multiple' => '4', 'navigation' => 'UDBG', 'display' => ['form' => TRUE, 'query' => TRUE, 'sort' => TRUE, 'tabs' => TRUE], 'js' => ['prefix' => 'PME_js_'], 'dhtml' => ['prefix' => 'PME_dhtml_'], 'cgi' => ['prefix' => [...]], 'language' => 'en-US,en;q=0.9', 'fdd' => ['CR' => [...], 'gene' => [...], 'FBgn' => [...], 'cg' => [...], 'vector' => [...], 'shipdate' => [...], 'plate' => [...], 'well' => [...], 'comment' => [...], 'sgRNA' => [...], 'sgRNA2' => [...], 'PCR5p_L' => [...], 'PCR5p_R' => [...], 'PCR3p_L' => [...], 'PCR3p_R' => [...], 'intL' => [...], 'intR' => [...], 'amp5' => [...], 'amp3' => [...], 'seqF' => [...], 'seqR' => [...], 'ID' => [...]]] )	...\construct.php:376
3	0.0014	411256	phpMyEdit->execute( )	...\phpMyEdit.class1.php:3402
4	0.0137	457704	phpMyEdit->display_record( )	...\phpMyEdit.class1.php:3202
5	0.0140	458568	phpMyEdit->display_copy_change_delete_record( )	...\phpMyEdit.class1.php:2531
6	0.0150	479168	phpMyEdit->display_change_field( $row = ['qf0' => 'CR71790', 'qf1' => 'BEAF-32', 'qf2' => 'FBgn0015602', 'qf3' => 'CG10159', 'qf4' => 'gRNA_int200_PM37_p1', 'qf5' => NULL, 'qf6' => '', 'qf7' => NULL, 'qf8' => '', 'qf9' => 'CATAACCCAGAGATGGGCGCCGG', 'qf10' => NULL, 'qf11' => '', 'qf12' => '', 'qf13' => '', 'qf14' => '', 'qf15' => 'CATGGCCAGCAATTTGAAGGGCAACAATACGACCAATTTGTGGACGCATCTGCGGCACAAGCACAGGGACGAGGTGCTGGTCAGTCCGGTGCAGCAACGTCCCCAGGCCCGGATGATCCCAATCATCAAGAAAGGTAATTCCAGCGAAGTTCGTATGGCGGGCTATCGACCCTTGGGTCGGCGGGAAACTATCGCCGGCG', 'qf16' => 'CCCATCTCTGGGTTATGATATTTTTGTTATTATCCGGTAGCTGTACCAATATGTATCGTATTAACCATCGCTTCCATCCTTTGCATTGCAGACAAAACCGTGCGGATTACCAAGGCCAAGACGCTGAGGGAGCCGCTGCGTGTGGCCAAGCCGAAGATCGAGTCGAACGTCGCCAATTATCTGGGCGAGGCGGGCGCCCT', 'qf17' => '', 'qf18' => '', 'qf19' => '', 'qf20' => '', 'qf21' => '5478'], $k = 10 )	...\phpMyEdit.class1.php:1102
7	0.0150	479216	htmlspecialchars( $string = NULL )	...\phpMyEdit.class1.php:1168

left_200bp	right_200bp
CATGGCCAGCAATTTGAAGGGCAACAATACGACCAATTTGTGGACGCATCTGCGGCACAAGCACAGGGACGAGGTGCTGGTCAGTCCGGTGCAGCAACGTCCCCAGGCCCGGATGATCCCAATCATCAAGAAAGGTAATTCCAGCGAAGTTCGTATGGCGGGCTATCGACCCTTGGGTCGGCGGGAAACTATCGCCGGCG	CCCATCTCTGGGTTATGATATTTTTGTTATTATCCGGTAGCTGTACCAATATGTATCGTATTAACCATCGCTTCCATCCTTTGCATTGCAGACAAAACCGTGCGGATTACCAAGGCCAAGACGCTGAGGGAGCCGCTGCGTGTGGCCAAGCCGAAGATCGAGTCGAACGTCGCCAATTATCTGGGCGAGGCGGGCGCCCT