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CRIMIC CR71791

Gene kat-60L1

CG1193

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	III:5781762..5787465	83C3-83C4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACCCTTCGTTCCCACCTACC	CTGCCTATTTGTTCCCATTACTATGCAC

Images

Construct Design Sequences

sgRNA sequence	ATATTTCTGCCAGTCATAATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAGAGATCTGTGGGCACAAGAAATCCACTATAAATTGGATTACTTCCGCATTTGAATTCCTTGACCTGGCAGTTGGTAACTAGCTCGTTGGTTGGTCAACTATCAACCCAAAGTATTTCCTTTGTTGCATACAAGAAAGCCACCGGGTACCTTCTTGACCCCATCCAGTTTCCTTTCGCGAGTATATTTCTGCCAGTCAT	AATTGGCATTTTGCTGTAATTTGCATAGTACGAAATGTCTTGTGTTGTGCCAATGGGATTAGCATAAGCACCCCTTGGTGACACGATTTCAAGTGGCCCATCATAACTACGAGTCCAAGGAAATCCGCCATCACTGGACTTTCCCATGCCGGGGTTTTTCCACGCTCAATCCGGCCTTGTAACACTTTGCCAATCAATCA

 

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