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CRIMIC CR71793

Gene Lcch3

CG17336

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:15925105..15928529	14A1-14A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTGCATGATGGATCTGCACTACT	GCTGACCGTGTATCCATCTG

Images

Construct Design Sequences

sgRNA sequence	TACTAGCTACATATGTAGAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAATTGCACTGTGGAAATTGAGAGCTGTGAGTAGACTCCTGAACTCGTTTGGTTGTCTAGAAATATTATTTACTTACCTATTTAGTTGTTGGATTTACCTGATATATTTTATGATAGTTTAGGACTTACACAAGATTTATTATGTATTATAAACTAAGACAGATATGCTTATAAATAGGTATCAAATTAGCATTCCTTTC	TACATATGTAGCTAGTATAACACAGAAGACAATTAAAACAATTCCAAGACAGATACAGACAGGTTAAAGATAGTTAAAGTTAAAGGTTTCTATAATCAGCTCGATCGAGTAAAATCTTAAGTTTGCGAGTATTACTATATAACTTATACTATAATATAATATATAATAACCTATTTTTATCACTAATATCTAAAGTTTAG

 

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