CR71795

CRIMIC CR71795

Gene CG17328

CG17328

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	II:16299943..16301765	35F1-35F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATCAGCTGATAGGAGTTTAGAGC	CCAACGATGCATACCAATACCAAAC

Images

Construct Design Sequences

sgRNA sequence	TTTCAATGTTAATTGGCATATGG
sgRNA2 sequence	CACTATGCTGCACCACACGGCGG

left_200bp	right_200bp
TTTTTTTTGTTGATTTCTATAAAATTGAAATAAAACATGGACTATAATATCCACAAAATATGCAGAGTTTGCCTGGAGGAACTACATCCTGTAACTTCGATATATAGTACAGACTTTGCTATGATGCCCTCCGTAATGTTGATGCAATGCGCTAAAATAAGAGTGGGTTCTAAAAACAAAATATTTCAATGTTAATTGGC	CGGCGGCACAAAGACTACATTATTAGAGTATAGCTACGAAGATTTATCTTAATCATAATTATTGTTTTAGATTATGTTTTGATATTGAACACTTTTATTTAGTTTGTCTATATAAAATGGAATATTTTGTCATGTTTGCTTTTATATCTTTACAATAAATACATGTAGAAATTTGGCAAGCACAGCTTCTATTAAGATTA