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CRIMIC CR71796

Gene Bre1

CG10542

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_kG4	III:5789831..5794192	64E8-64E8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATGTGCATTTCGTGTTCGATCCC	CCCTTCAAAGCAAAGCGCGACTTT

Images

Construct Design Sequences

sgRNA sequence	AGTGTATAAACCCGAGATTAAGG
sgRNA2 sequence	GGGTGTGCGGTAGTGGTGTGTGG

 

left_200bp	right_200bp
TTACCGCTAAGCACAGACATATAGTTATCGATAACGTGAACGCAAATGCGAGGGAGTATTGCGAATAGTGTTACCGTGCTGCTGTGAAAATTCCCAGGTGTGACCGCAGCCCGCATTCGATATTGATGAAGCCAGGCAAAACGTAGAAAAGCAAAAGAAAACCTTAATTAATGTTAAGTTAAAAGTGTATAAACCCGAGA	ACCACTACCGCACACCCAGCATTCATGCCGGATTATTATTCATTATGCATATATTACCTACGTACTAATATTTGGTTAAGTAGTCTCCGAATAACTTTCCCCCACCAGATTTCCCATGGCAATTATTTGAATTCGTTCATTCTGGCGCCAAGCAAAAGCATCTCATTATGAGTGCCGCGAGATCGTGACGCGGTTTTTAT

 

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