CR71797

CRIMIC CR71797

Gene Phae1

CG16996

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:12109417..12110664	33C4-33C4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAAGAAATACTAACAGGTGATAACGCG	GACTCAGTCAACAATGAGAATAGATGC

Images

Construct Design Sequences

sgRNA sequence	ATTTAAACGTGCCGCGATAAAGG
sgRNA2 sequence	AATCAAATGTGTGTCCCTAGTGG

left_200bp	right_200bp
TTAGATAAACGAGCGTAGGTATTTGCCCATCCTATCGGTGGAACTTGACGTACTTCTGTCAAAGAGTCACTGTTCGGGCGTCGCTTAATCTCAAATTATAATAAGTGGTTATCTGAAGAACACACGGTCCTTAATTGAAAGCCTTATCTGCTGGCGCTGGCAAAATTTTGGAATGGCGAATTAATTTAAACGTGCCGCGA	TAGTGGCGGTCACTTAAATGTGGGACTTTCCTTCGCATCACAGCTACTATATCAAACAACGAGCTGTTTTTTAAAGTTTATCTTATCTCGTAAAGAGAGAGGAACACGGTGAGCAACCAACGAACAGATTTATATGCTTCACATATTTTCGGGCTGTAAATAACAATTTCTTACATTGCACAATAACAGAATAACGAACC