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CRIMIC CR71800

Gene kappaTry

CG12388

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:11337797..11338733	47F3-47F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTGGACCAAAGTTCCCAGTGTTG	TATTGCTCCGTACCGGATGTTCTC

Images

Construct Design Sequences

sgRNA sequence	CTCACACCCGTGCGAATCGATGG
sgRNA2 sequence	ATCGATATGCTATCTTTAAGTGG

 

left_200bp	right_200bp
AAGGATTAACGTATTATTTACACCAATAATGTGTCACTTGGATTGACATTATCTTGGTCGGACCCTGTGCTCACACCGCTATCAGAAGCTATCTTAAGCACCGACCATTAACTCAATCGGCATGTTCATTGAACGCAACCTAGCTAAGTCTAAACTGAAATCCGTAATCAGTTCGATTGGAACCTCACACCCGTGCGAAT	AAGTGGTTACCGACGAATAGAGCTACCTAATTTAATAATAAAACCGTAATCAAACAAACAATATACTACGTTATCGTCTGTATTGGTCATTTCTTACGTAATTCGAAGATATAAAAGGTTTTGAGTTGATTGAGGCTAGTTACAAGACGTTTGAATAGTATTTTTGCCTTAATATTATTACAGAAAGTCGATTTTGCCCA

 

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