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CRIMIC CR71801

Gene ranshi

CG9793

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_kG4	III:8728367..8729693	85A9-85A9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATAGCAATTACAGGCAATGCTAGAC	GGAAAAGGAGCCAGAAGCAGAG

Images

Construct Design Sequences

sgRNA sequence	TTTATCGGCTCAAAAATGGAAGG
sgRNA2 sequence	AAGAAGCTCTTAGTTGAAAACGG

 

left_200bp	right_200bp
GCAAATAATTAACAAACCAATTGTAATATGTAGAACTTAATATTGAATAATAAATCAAAACTTCAAATCCCAACTTATGAGCATTTATTTCATCTGTAAAAATTGCTTCCATGCGAATGGCAAACGGAACAGCTGATTGACAAGCATAGCATTGTTTACAAAAAGTTTGGCGTTTCAAACTTTTTTATCGGCTCAAAAAT	AAACGGTTGCATTACCAAAATAGGAATTTTTACAAAAAAAATCAAAACGGATAATCAAATTGTGAACTATATTTGGCTTTAAAGGTTGAGATGGACTCATTAAGGTCTAGTTTGTACTATACATTAGAGTGGATGTTTCCAGAGCAGCCAGGCCCAAAGACTCATCCTGCTCCTGCTCCGCGGGCAAAGGCACTTCTATC

 

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