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CRIMIC CR71804

Gene Brd7-9

CG7154

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	II:7782865..7786061	28C3-28C3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGGAACGGTCACACTGCTC	TTAGACCTCGTTGCAGCTGAG

Images

Construct Design Sequences

sgRNA sequence	TGTGTGAGCGAGATGGCCTATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGCGTCTGTTTTTCGCCAGCGGATTTTTGTTTTTAATTATTTTGCTTACCGTTTTCACCGATTTGCCTAATTGTTCGACAACTGGGAGCCTAAGATGGGCTCCTCCAAGAAGCACAAGAAAAACAAGTCGGAGCGACGCGAGAAATATGAAGGTGAGTGAAATGAAAGTGCTGTGTCTACGTGAGCCCCTGTTGCCATAG	GCCATCTCGCTCACACATTTTGCCGCTTGTTTTTGAGCATGCCTACACTTAGAAAAAAATGTGTGTATATTATTTGTCACAATTAATACAAAATTTATGGTTAGAAATAATCTACGCGTCTAAAGGTAGTAATAATGCTATTCAGCACCCATTATAATACCTATACCTTATATAACCTGGCTTTCACCTATATATATTAT

 

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