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CRIMIC CR71809

Gene CG1827

CG1827

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_kG4	II:9589050..9590443	45F5-45F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGTTGTGTCAGTGCTGTGCCTAC	TTGGTAGTCATAACTATCGAAGGAATGG

Images

Construct Design Sequences

sgRNA sequence	GTCCCTGTTCTGCCGGAAAATGG
sgRNA2 sequence	GTAAATATCGTGGCGTTGTGAGG

 

left_200bp	right_200bp
GGATAGGACTTTATCGATATTAATAGGTGGTTTTTTGCCTATATCTTAATCTATGTTGAAACATTTTCAAGTTTTAAAATATTGCTTAAACCAATTTGAAAATGTGTATGACTCTTTTAGAGGGTATATCAAAACATCAGATAAGAAAGCAAAACAGTGAGGTTAGACTGCTAGCATTTAAAAGCACAACTCGACCATTT	GTGAGGTGGCGAAAACTGTAACGTATTTCAATAAAATTTAAATTTCGTGGAGTTCTCATCTGCATTATAAATTGCGGTATTTGTTTGTCCCTATCGGCAGCAAGATAGACGGGTAGTTTTACCGTAAATTAGCGGTATTAAGAATTATCGATATGTAAGGATCCTTACGGAAAATAAATAAATAAAGTATGAGAATAGAA

 

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