CR71814

CRIMIC CR71814

Gene Cln7

CG8596

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:7341884..7345183	65F5-65F5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACCGATTGCCTTTATCTTCCGCA	CACATGCTGATGCCGACGACAATT

Images

Construct Design Sequences

sgRNA sequence	CGGCGGGCAAACTCCATAATTGG
sgRNA2 sequence	CGATTTCACATGCGTAACGACGG

left_200bp	right_200bp
TCTACTCTGAATCATTAATAGTTACAAGACTTTAAAAGCGTGTCTAACAACTGACACATTCATTCACAAAAAGCTCCATAAAGAAAAAAAAAACTCATTCGAATCCATTTACAGAATGACTCAGTGGCGGTGAAATCCAGCAAACACACATCCATCGCAGCCAACCAACCCATCAAACATCACAGCCAAGCAAACCAATT	CGACGGCTGGCTATGGAGCATCCGTAGATCTAAGACACATCGTTGCAATACTGCCCCTTAAACTAGCTTTAAAATACAGTGTTTATTTTTGCACAGTTTCGACTGATTATGCGTTTATTGGTACTACGGTTGAATGTGTAGAGGTTGCACGTCGTTGTCTAGTTCTTTTTTTTTTTTGGCGTGTTTTGCATATAGGGAGG