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CRIMIC CR71818

Gene Oat

CG8782

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	III:19647280..19649851	76B11-76B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGATGTCATGCGATGGATTAAGACTT	ATCCGAATAGAAGGCACTGCACAG

Images

Construct Design Sequences

sgRNA sequence	TGGGTCTATAAAGTCAAAAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCCCCATGAAGTGGACGGAATCTTATCAGCAGTCAGTACATGTATAAATTCTCACTTTACCTAGTGATTCTACATTTGTCACAGGGAATTTATTTAATAATTGTACTTTACTTGTTTTTCAATTTTTGATGCCGGTTTTATTTGGTTTCTCTTGGGTTGCCAGGGCCAGATTGTCAGGTTGAGTGGGTCTATAAAGTCAA	AAGAGGTTCGTTGATAACAAATCGAGGGTTCGATGACTTGTTATTGTTTGTTGCAATTTCTAAATTTGGAAATTGGAGGGGAATGATTACGGATATGCCAATGGAAAAATATTAACGGTTTATTTTTGGTTGATCCAAATGGTTATTGTCCTACTTGGGCAGTTTAAAAACAAAACGTGACGTAGCAATTAAATACAATA

 

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