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CRIMIC CR71839

Gene Ance-4

CG8196

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_kG4	II:9039728..9042142	45A1-45A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACTAGACGATCCGCACCTGATTC	GTTGCCCAATTCCGGAACGCATTT

Images

Construct Design Sequences

sgRNA sequence	CAGCAGCAGGCCCATCTCCTCGG
sgRNA2 sequence	AATTTCATTTATTAAGGTGGTGG

 

left_200bp	right_200bp
GCTTAATAAACAAGACATCATGGCATTCCACAATTCCACTTTTGTTCTCAATTATTGTGGCTCGGCCTCCGGGCGAGAATCTCTCGCTGATTAGGCTTATCAGCCGCCCGGGAGAAGCTTTAAATGCCAGCTCCACGGCCGGAGCACCTCAGTTCGAGCAGATCCGGTGTTGAGTGCGAAAGTGAAACCGATTCCCGAGG	CCTTAATAAATGAAATTGCCTTCTTTAATTGGATTTTGAACTTTAACCGCCTTCGAAAAAAGCCTTTTTCAACACTGCCGCGAATCGGTTTAAACGGGTTAATCTGACTAGTCATCTCCCTCGCACTTGTGTACTGGGAATTGGCTTAAGGAGCGCATGTGTTGGAGGGAGATGACAGTAGCTGTTGCTTAGGCGCTAAA

 

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