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CRIMIC CR71845

Gene nmdyn-D7

CG8362

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_kG4	III:9679939..9681502	85E4-85E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTCCAGCTTCTTAAAGATGTCGC	GTTTGCATCATCCTCTATGCCC

Images

Construct Design Sequences

sgRNA sequence	CTTGCTGTCAAGCGATAGAACGG
sgRNA2 sequence	AGCTCCAGGTTGGAGTCATCTGG

 

left_200bp	right_200bp
TTGTTTATGGGTAATCCCCTAGAATTTGGGTTTAAAATTAACATAGGTATTTTTTTCTTTAAATTTTGCGCCCACTTAAATGAATTTTGTTATGTTGGCAACACTGTTGCTGTGACAACGCGGGAAAGAAGAAGCACAATTGAAATGCAAAGCAAACAAACAGTTATCACTCGAAATAAAGGACTTGCTGTCAAGCGATA	GACTCCAACCTGGAGCTGCAGTATATGTTCAAGATAATCGACTGATCTTAGTTTAGTTTTTATTTGTTTTCAGTTAAATGCATGTTTGCCTGTGTGCGTTTATCAATAAATTACAAGTGCAATGAAAATACTAGACCGTAGAAACTTCTCCTATATGCTCCCGCTGCGGCTGGTGCTGGGCACATCCCGGTCCTCCGCTT

 

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