CR71851

CRIMIC CR71851

Gene CG12376

CG12376

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	II:8701835..8704502	44E2-44E2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	multi	CTTGTTGGCTTTTGGGAACAGCTC	TGTCTGCGGCTTTCCTCCAATT

Images

Construct Design Sequences

sgRNA sequence	AAGAAACATAACTAAAATTGAGG
sgRNA2 sequence	TTGGTGATATCTAGGGAAAAAGG

left_200bp	right_200bp
AGGAGGCCTGATCTGCTACACGAGTCCCTTCAAGATGAACGCGTACGAAAGTGTTCGGGACATTTTGTTTCTTATCTTCGGATCAATGCTCCTTCAGTACTTCCTGGCCAGCAGTGCACATGTACCCGAAACGTCATTCATAGGCAAGCTTTTCGCTTTGGAAGAAATCATTATTGTTACTAATGTTACTCCTTCCTCAA	AAAAGGTTTTCTATGGAAATATACAAAATTTTGGGTACTTAATTCATTATAATAGTAATGAATAATGATAATCATAATTAATAAATATACATTTTATAATGTAATGTAATGTAATGTTTTATAAAATGAATTTCCCTGCTAAGGTGGTAATTTCAACTGTGAAAGCAGTTATCGAGCGGCATCTTAATCATAACAGCTCA