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CRIMIC CR71868

Gene Shi

CG18102

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:15892116..15906716	13F18-13F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTATAAGCCTGCGCTGTCTCTCAT	CATTGACATTAAGACTTTGGCCTGC

Images

Construct Design Sequences

sgRNA sequence	CATAGCTTGCAAATAAGGGGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAGAAAACAATGGCTTACGCCCCAAAAAAATGCAGATAAGGCCAAAATGATAGTGTGTCGGCGATTTAAGTTTTTCCCCACTCTTGCCCAACGATTGTGTAGAGTAACTCACATTCTATATGTTTACTTTCGAATATATTTGTAAAAGTTTGACCTCCTTTAGAGTCGATTTATTTATTTTTCATAGCTTGCAAATAAG	GGGGGGGGGGGGTGCGCTGAAAAAGAACATTAATCAATATGCCCATTCATGTATTTACAAACTGCATTAAGCCATGTAAAGTTTGTGCTTACATAAGTCCACTATGTTCAAAATAGTCCAGTTAGATTTCGCATGCGCCGTGCTTTATATGTATGTATGAATGTATGTATGTGTGTCAATTAGGGTGGTTACCCCGCCCC

 

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