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CRIMIC CR71876

Gene slow

CG7447

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	III:4384442..4403181	64B3-64B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGACTACAAAGGTGGATGCGGATC	GGCGACTGGCAATTGAAAACTCGT

Images

Construct Design Sequences

sgRNA sequence	AAGACGAGGCAAGACCAGTCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGTCTCAGTTCGGCGGTTCAAGGCTGTTGCAGCCATAAATATGACCGAAAAGCCGGGCTGGTCATATGTCTTACCTTTGGCCCAAGATCTGCGGCTTATCGCGGCGTACATAAATTATTTAAATATTATGACATGCCGCGTTTTCAATGTCAGCAGCTGCGCTTCGTGGCTAATTCGAGCCGCAAGACGAGGCAAGACCA	GTCCGGACCAAATGCCAaCCAACTGCTGGCTGGAAaTTAGCCCCAAGGCCAAGTGGCCGTGATATTTTCAGATTTTTCTCAACAAGCACTTCAATTGTCGTGTGTCAATTGCAGCGAATCAATGAGCGTGCACTTtGGAAACTATTGCAATTGCAGCTCCCTAAGTGTAACAAACAGATCTTCGGTTAGATATGGCTTGT

 

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