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CRIMIC CR71905

Gene Nepl19

CG14523

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:28878024..28880122	98F1-98F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTGTATTCCAATCACGCACTCCC	GAGTACAAGTGCGCCATCTACTGA

Images

Construct Design Sequences

sgRNA sequence	CGCTCTCGGAGTGTTCGCCATGG
sgRNA2 sequence	AATGATGTCCTAACTAATCCAGG

 

left_200bp	right_200bp
AAGATTTCTCAACCGGAAAATATATACAATATATCATATTAACAAATTGAAATTGAAAGATGGATATAACAATTTTAAGTCGGATCATCTACTGCGTTCAGATTTTTCTCAGCGCAGCTTAATCTCAATCTCAGTCGCAGAGAGTGGGTGCGGAAGTGCGTCTTGTCGAGCTGCCCGTTTAGTCGCTCTCGGAGTGTTCG	TTAGTTAGGACATCATTTAAAGTACTCTGAACTACAATAAATTCTACCCATAATAAAAACTAACACTGAAATGTTGGGTCATTGAATGGTTACTTCTCGACAATTTAATATACACATATATATTGTCAACAGCAATTATCAGAGCGCATATATAGCCATGACCGTGTCTATATGTTTCTACGCACTCTCATTGATAAAGC

 

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