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CRIMIC CR71920

Gene per

CG2647

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:2685580..2692780	3B1-3B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATCCGCATTGTAGTGGTTGG	TTGCATGGGTTCTGGGCATC

Images

Construct Design Sequences

sgRNA sequence	GCTGGAGAACCTAAGCACCATGG
sgRNA2 sequence	TCTGCTGCTCGGATCATCCCAGG

 

left_200bp	right_200bp
CTCAGTAGTCACGGAATCTCCTCGAAGGAAGGACATACATAGATACATAGGTTCATCATAGAAGTGAAATCAGTATTGTTACAGTTTAGTTACCCCCATTCAAGGTCCTATCAAGGATAACTGAAGTGCTAATGGTCATCATACGATTTTCCAGAATCTCGCACTAGTCAACCAACTGGGCAAGCTGGAGAACCTAAGCA	ATGATCCGAGCAGCAGACACCGAGGAGCAGCAGGGTCCTGGAAACGAGTGAGCAATTGCCTCCGGTCTGGGCACCGAGCACCAGCCAGTCCCACCAGTTCCTATTCCTAACTCCCCCTTCCATTCCCTTCAAGTTCTTCGAATCAATGCGAACGCATCCGTGGGATGTCACCATTCAGTATCCGAACAAGTAACGAAAGC

 

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