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CRIMIC CR71955

Gene sgll

CG31472

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	III:7810532..7811912	84E1-84E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCTACACTAAGCGCAAACAAG	CCAAAGCGTACACCACAGCTTTTG

Images

Construct Design Sequences

sgRNA sequence	AGTTCCACAGAGTTACCAGCAGG
sgRNA2 sequence	TGTTATGCGCAGGATAATAGAGG

 

left_200bp	right_200bp
CGCAGAAAAGCTCATATCTGACCTTGAATAAGTACGTGTAAATCTTATCTGCGAAATCGGCAATAATCAGTAGGTAAACCCCACAAAAACAGTGAAGTTGCTGCAAACAATTCGAAGGATGAGCCAAGGTGCCTCGGAGGTGCCACTTTCGGGTAAGTAACATTAATAACCTAATATTATGTTGTAATAAATGGCCTGCT	TAGAGGAACGTTTAAAGCTGCTTCAAAATAAATGTTAAGACATAACCAACAACTATGTACATCTGTACAAGCTTAACTACCAAATACAAATTTAAGATTGCCTTTAAATATTGTCATAAGGTGTAAGTGAAATTACAATTCCTTTTTAAATTTGAAAGTACACTAGCTAAGATCACATCCTAAAATATGGCCTATAGCCC

 

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