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CRIMIC CR71970

Gene CG3857

CG3857

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:1937700..1940040	2B16-2B16

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCTATAAGTCCAGTGCAATTGTG	GAAGAGCGCAAGAAGAGGGTTCTT

Images

Construct Design Sequences

sgRNA sequence	ATTCGGAAGCCGGTTATTTGGGG
sgRNA2 sequence	AACCGCGTACTTACAATCTGTGG

 

left_200bp	right_200bp
ACCGTTATTTGTTGGGCCAGCCGATTGTTGCGTCTGAAATTAAAAATGTTGTCCTTAATTTCGTATTTTTCGCTTTTTAGCTATATTTCCACGACATCTGCGATGGTTATGTACATACCAGTGCTGCGAAACAATGTATTAGCGTTGACACCCACGAACGTGTTCAAACCGATTTAAATTTAGTCAAATATAAACCCCAA	ATTGTAAGTACGCGGTTGGTATAAAACTATGCAAGTGGGTGAAGCGGCTTGGACTGGGATACGCAGTCCTGAATCATTCAAGTGATTCAAGTTCCAGTGGCAATGTTTACCTATCCTTTTATTAATGACACGTGCAGTGTTATGGCCAATATGTTTAGCTGAATACCGACCGACATCAAGTCTTTTTTTTAAAGGAGGTG

 

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