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CRIMIC CR71976

Gene CG10031

CG10031

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:3698451..3699092	24B3-24C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTTTCAAGTGATAGGGTATCGC	CTAACGAATGGGGTAAGCTTTGG

Images

Construct Design Sequences

sgRNA sequence	GCAACGACTGTGAACTGAACTGG
sgRNA2 sequence	TTTAAAATAATTACTTGAAATGG

 

left_200bp	right_200bp
AATGCGTTCCAATTATGTGCTATCTGTTTGCTTTGTTGAAATTATTTAATTAATCTCGCCAGCGTTCAACACGGCGTATGCGCAATGCGAGCTTAACTCACACATGCATGAGCAGAGAGTGAGCAAATGATTTAATTTGAACGTAATTGGTCGTCTAAGCACAGCATATAGAACTGGAATATAGAACATTCGGACCAGTT	AAATGGGTTTTTGCTAATGCGAAATATATACGTTTAATAATTAACTAATTACAAGTAAGCAGTATATCAAATGTTTATCTATAATTGGAATTAAGAAACTAAGTGCATTATTTTCCACCTATATGCGCAATTTTGAAAATTACTTAATTATTAGAATCGCAAACGAGCGATTATTAGGAACATTTCTTAACCAGACAGTT

 

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