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CRIMIC CR71977

Gene Tmtc4

CG5038

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:15351225..15353926	88F1-88F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGTTGCAAGCGTTAACG	CATGTAGCGCTTAGTTCTAGAGTTTAG

Images

Construct Design Sequences

sgRNA sequence	CTGGCATCACTATTCAGTGAGGG
sgRNA2 sequence	TTGGATCCATATTTGGATGGTGG

 

left_200bp	right_200bp
GTGGTTTATTGTTATTACTATTATTTATACTTTAATATCAAGAGTATGTATAATTATTTTAATATGAATACTCTAAAATGAGCAGATTTGATAGATCTATTTAGCGTAATTTCAAATCATTTTATAACCATATATTTTTCGATGCATATATTTAGCTAAGCTGCACACATTTCTATCGCTGATCTGGCATCACTATTCAG	TCCAAATATGGATCCAACCAAAATCTTGGTTGTTTGCAAATAATAAGAGGTATGTACACTTCGCAAGCTGTAATAATCCCAAACGAAAGACGAGGTTGTGGGTTAAATTACTTATTCATAAAAGACATTATAACTAGTTAGTCTAATTTAATTTTAATTCCGTGCAATATTGTATCTATATCATACATTCATATCCCATC

 

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