CR71979

CRIMIC CR71979

Gene CG10053

CG10053

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	III:7247090..7248140	84D3-84D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGAACTCAATCTTCATAGCAGAG	CATTCGTGTTTAGTGTTATCGAAAGGACC

Images

Construct Design Sequences

sgRNA sequence	GACATCTTGCAGACTAATGAGGG
sgRNA2 sequence	ACGAGTGAGTCCTGGACAGTTGG

left_200bp	right_200bp
ATCATTGTTATTATAAATTATCGAAATAATGCTGTAGATAACTATGCAATGGTCACACTTAAATGAGAAGTGTAAACAAAACAAACAGATGTCTGACGAGGAGGAGGATTATATGTCCGACAAATTCCTGGTTGGGTGAGATTACACTCACACCTAACTAACAACCAACCACCTTATGAAAAACTCTCATCCTTCCCTCA	GTCCAGGACTCACTCGTGACGAACACTAGCTCCAATCAATAAAATAAAATAAAAAAACTAGCTTGATTGCTTACACATAATTTCATAAAATCAGCAGGTTTAAAATATTTTACAATAAGTAGTCAAAAAACAGTAAGCGGTGTATCTGGTTTGGAGGAATGTCTATAAATGAGTTGTAATCCAGTTAGTGGGAGCTACAA