CR71983

CRIMIC CR71983

Gene CG13748

CG13748

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:8940415..8941136	44F9-44F9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTATGTTTTACGATGATAATGCTCACAACAG	ACGGACACACCCAGGAAAGTA

Images

Construct Design Sequences

sgRNA sequence	ATTTAATTCACTTGATCACTTGG
sgRNA2 sequence	TCCGGATCGGAATGCGTATGCGG

left_200bp	right_200bp
TGGATTTTCAGTACAAAAGCGGACTCTTCGAAAGAGTAACCAAAGAGCGTTGGAGTCGAACGGAAAGCTCTGCTGGCTCTTAGATGGGAGTATTGTCTAGTATTCGCTTCCCTTCGCCCGCTTCGGACTACGAAGTGGCACCATATAAATCCAAAAGCTGGACTGCAGCGCCAACAGTTGCGTGAAGACAATCGCCAAGT	ACGCATTCCGATCCGGATAGCGATCGGTCCTCCGCGACCGATGTGTTGCCAAAGGCGGCTGCTTCCCCTCGAGCTCTGCAATCCAGCGAACCAAAGGCAGCATTCTCCACACCCAAACTCACACACAAACATCACAATCCATGCGACTAACCGTCCCTTCTTCTACCTAATATTTATGTAGTTTCTGTCATTGAATCCAT