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CRIMIC CR71994

Gene Mtpalpha

CG4389

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	II:9573563..9576769	30B12-30B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGACAGGCAAACAGGTGCG	GATAGATCGATATCAAACTGCTGTGTG

Images

Construct Design Sequences

sgRNA sequence	TTTCTCGCGATTTACCTGTGTGG
sgRNA2 sequence	TTTCGTTAGCGGCTCTACGGAGG

 

left_200bp	right_200bp
CAAACGACTGGGCATCGTGGATCTGTTGGTGGATCCCCTAGGTCCTGGACTCCAGCCTGCTGAGCAAAACACTATCGAGTACCTGGAGAAGACCGCCGTGCAGGTCGCCAACGATCTGGCTAGTGGTAAACTTCGCGTAAGAAATACTAGTTAATCCCTTAAAGTCTTCATCATTAATTTGTTTTCCACCGTATCCACAC	TAGAGCCGCTAACGAAATTTAGCTTTTAGTGCGAAGATAAGTCGTGTCCTTGTGCATTTCTCTCAGAAAGGAAACCTATTGTGGTCCATCGCTGCCTAAAAAATGGGGCAAGGCCCAATAAAAAGCCCTCTGTTGAAGTTGTCGACAATTAAATTAAAGTTTACCGTGTAATTTTCCATACTTTCATTTGTTTATTATTA

 

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