CR71999

CRIMIC CR71999

Gene Idi

CG5919

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:21270126..21271408	93D2-93D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATACCACTTGGCCAGGCGAGTTT	CATACAAACACATTAGTTAGGCTATGAAACATA

Images

Construct Design Sequences

sgRNA sequence	AACTTCGTAATACAGCGCGCGGG
sgRNA2 sequence	GCACCAGCTGGAGCAATTTGAGG

left_200bp	right_200bp
TACCATTTTTCGGGAAATTGGTTAAACCGTTTCCTGCTTATTTACAACTTTTTTTGTTTCGAGAATGGGGGCGAAAAACTGATTATGAGCAAATTGTGTATCACTGGGACCAGGGCTACCACTACTGTTCCGGAACAGCTGTTACCTCGTCAGTGTTGCAAACAGTTCGCGCAGAAAGCAAACGAGTTGGCAGACCCGCG	TTGAGGACTTGAGTAATATTCAGCGCTTTTAAGTGTGCTAACCAACCAAAAATACACGTAATGACGTCTAAAAGGGGCTTTTGTTCGTTTGGGTTTCAAATTCGCTTTATTCAAATGGAAAATAGTGTGTGTCTGTGTGCGGACAATGGGCACAAAGCGGATTTTATGCAACCTCCGCGGGTCGATCAGTTCAGTACAAC