CR72010

CRIMIC CR72010

Gene Cyp303a1

CG4163

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:16294183..16296969	35F1-35F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACCCATACTACTTTTCGCAACCC	ACTAAGTGAAAACTTCGTAGACTACAAATC

Images

Construct Design Sequences

sgRNA sequence	TTCTACCAGTTACGCAATCTTGG
sgRNA2 sequence	TAACCTTATTCACCGTGTCGAGG

left_200bp	right_200bp
ACGATATAACTTTAAAACGACAAATCAAACAGTAGGAAAAGCTTTTCCACAAGACCTCTATAAAAGCGACGCCCAACCAACCGAGCGGATTCAGTCGATTTGAGTGAACGTCAGGAGCGGGATCGAACTGTGGACGAAGTCACCAGGAAGCAGTGGATAGCTTACCAACCCGAAAGTCCAGCTCGCAACTCCAGCCAAGA	CACGGTGAATAAGGTTAACAATATCACTGAAACATCCTGCATCTTCCGCTTAAATCGAAACATATGTATATTATCAAAAATTGATGGGATGAGCTGAAATAAGAATTTTCATTTAAAGCCCCTTGCACTTCACGTTCACGTTCAATAAAATATAAATCAGTTTATATTACACAATTCTCGTTTACATTGTCTAATTAAAC