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CRIMIC CR72014

Gene Cyp9b1

CG4485

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:7127838..7129808	42F2-42F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTACGAGATACCGGTTAGTTAGC	ACGTTTGTAACCAACCAGTGAGAG

Images

Construct Design Sequences

sgRNA sequence	TCAGTTAGCATTAATACATCAGG
sgRNA2 sequence	ACTTTATCGTACAATATGAGCGG

 

left_200bp	right_200bp
TTGATATCAAGTACTCGCAGTGTGCCTACAATGCCAATTTGGCCCCCAAATACTTAAAGCTACATTCATTAGATAAGAAAATAAATAAACAAATCGATGAGAGCTATGTTCTCACTAGCTTTTTCACAACTCAAAACCCAAAGAGAGCAAAACATATAGATACGTAGTGGGCACTCATTGCTTTCAGTTAGCATTAATAC	ATATTGTACGATAAAGTTGGAGAGAACGATACAAGGGAGTTCCGCGACAGTCAGATACCCTTTACTTAGCTGTGGGCATTCAAAGTCGTTTTGAATAGGAATTGACAGGATTTGGAGGATTCCGGTTAATAAAAAGACGGATTAGTTGATAAGAATAAAATACACAGACATATATTTTACTTTTGATTATGTTTCCATAC

 

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