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CRIMIC CR72017

Gene Cyp6a17

CG10241

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:14873953..14875739	51D1-51D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTAGCATTTCACCAGTTCCAGGG	CGATCAACGTTAGTAACAGCGAC

Images

Construct Design Sequences

sgRNA sequence	AGTCAGTTTGTATTGGACTCTGG
sgRNA2 sequence	TTAAAAAGGTTAGTTAAAGAGGG

 

left_200bp	right_200bp
TGTTCATATATGTGTATACAACCATTTTAACTGCGCATATGATTCCCTAATTATTATTTATCACATTGAGAACAAACCTTAAGAGAACTTGAACGGTGAGTTTTGTGTGTGAAAATGAGAGATTGGCGAAAAGAGCGAAGACCTCATACGCGAATGAGCTTGCGTTGTGGCATCCCACTTTAAAGTCAGTTTGTATTGGA	TTAACTAACCTTTTTAATGTGTTATGTGTTAGGCAATGTTTTGATTTGCTAGCAAACATCACATAATAAGGCATACACACACCGAAAGCAATAATATTCGCTTTATCAACTAGCTTCTGAGTACATTTGAAGAGATTAGAAGAGAACGTTCGCGTATAAAGGGGCCGTATCATTCCGGTTGGTAACTCAGTGTGGAGCGG

 

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