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CRIMIC CR72021

Gene stan

CG11895

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:10673345..10721138	47B4-47B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAGCAGAAGCTACCTCACACAC	CTCGCTGGCTAAATGTTTGTGTGG

Images

Construct Design Sequences

sgRNA sequence	TAGGACCATTAATTGCACTGTGG
sgRNA2 sequence	TCCCAAAAGTATGCTACGATCGG

 

left_200bp	right_200bp
agtttaaaaatgtaatttttatatatttttttttCcagcaggcgacaatttcaaatacatggcctacatttaagggcaaacgcaactcaattacccccgctaacctcactgactctccacattttcttgtccttttcagacagatggccatgcccgcagcaccgcccaccgatgataattgaataggaccattaattgca	gtagcatacttttgggagcacctgccataggggcatgcccgtcattataattgaaactttgtacatacaagcacccacttaattttacgtaaacccagcttagtatacccccattcgggcacagatatttatacgatagcctagcatttacataatttgaggcgccagttgcatttcgttcggaattgcataactcgcat

 

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