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CRIMIC CR72025

Gene ND-49L

CG11913

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:25358285..25360034	96D2-96D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCGAGACCTTGTGATGTGC	GCAACAGCTTATGTCATGCGTCAC

Images

Construct Design Sequences

sgRNA sequence	GTTCCGCCAGACATGATTACCGG
sgRNA2 sequence	TTTGACGTTCATACACAAAATGG

 

left_200bp	right_200bp
ATCATCGAAGATCTGCAAGGAATGATGGTTCAATGGATTAATAGGCAGTTTACAAAATAACATATAAATCTAAAAATAAACTGGAGATAGTAGTCGTTGCTCTTAACATCTTAGTAAGCTAAGGGTAGAAACATTTGTTTACATTTTTAATTTTCCAAAGAAGTTAGTATAGAACTAATATTTTTTTTAATACTCCGGTA	TGTGTATGAACGTCAAATGCCCAAAACCTAAATAAAACGGAAGCCTATTTATGCAGCATGTAATTTAAATAGCACTCGGAGAAAGTATCGAATTTATAGACAAATTTTTTTGGCCAATAAATTTGAAACCTTTATTCTAATAAATATAATTACACATAAAATATAAATCATTAATTAATTATTGAAAAATTCCCACTGAT

 

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